A2228
 |  |  |
 | Immunoblotting Chicken fibroblast cells were separated on SDS-PAGE and probed with monoclonal PA to Beta Actin (MO) AC-74 (Cat. No. A2228). The antibody was developed with Alkaline Phosphatase conjugate (Cat. No. A1293) and a NBT/BCIP substrate. Antibody concentration 0.5 μg/mL. |
Image
Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- A2228 - Datasheet (44 KB)
- Bulk Quote
- Similar Products
A2228
Sigma
Monoclonal Anti-β-Actin antibody produced in mouse
clone AC-74, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD00164531 |
Description
| Immunogen | slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Specificity | Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin's solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting. |
Properties
| antibody form | purified immunoglobulin |
| clone | AC-74 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | canine, guinea pig, rabbit, bovine, chicken, rat, sheep, wide range, carp, Drosophila, Hirudo medicinalis, cat, human, mouse, pig |
| application(s) | immunoblotting: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts |
| immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts | |
| immunohistochemistry: suitable | |
| microarray: suitable | |
| isotype | IgG2a |
| Gene Information | human ... ACTB(60) |
| rat ... Actb(81822) | |
| mouse ... Actb(11461) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Langlois, B., et al., LRP-1 Silencing Prevents Malignant Cell Invasion despite Increased Pericellular Proteolytic Activities Mol. Cell. Biol. 28, 2980-2995, (2008) Abstract |
| Cotman, C.W., et al., Differential regulation of inhibitors of apoptosis proteins in Alzheimer’s disease brains Neurobiol. Dis. 26, 165-173, (2007) Abstract | |
| Aiken, C., Proteasomal Degradation of TRIM5a during Retrovirus Restriction PLoS Pathog. 4, e1000074, (2008) Abstract | |
| Gimona, M., et al., β-actin specific monoclonal antibody. Cell Motil. Cytoskeleton 27, 108-116, (1994) | |
| North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994) | |
| North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994) | |
| Liao, J., et al., Direct reprobing with anti-β-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000) |
